ABSTRACT
Long-chain non-coding RNA (LncRNA) has more than 200 nucleotides in length and cannot encode proteins. It has a variety of biological functions. A large number of studies have shown that LncRNA is closely related to the occurrence and development of cancer. Researches at the level of molecular biology have found that LncRNA acts as an important regulatory molecule involved in the whole process of life activities and plays a regulatory role in various diseases and tumors. LincRNA-p21 is a novel LncRNA that acts as a translational inhibitor by targeting mRNA or by directing the chromatin site of a protein-binding partner. LincRNA-p21 is closely related to a variety of tumors and exerts its biological functions of carcinogenesis or tumor suppression through different pathways. In this paper, the research progress of the tumor-associated gene LincRNA-p21 was reviews
ABSTRACT
Long-chain non-coding RNA (LncRNA) has more than 200 nucleotides in length and cannot encode proteins. It has a variety of biological functions. A large number of studies have shown that LncRNA is closely related to the occurrence and development of cancer. Researches at the level of molecular biology have found that LncRNA acts as an important regulatory molecule involved in the whole process of life activities and plays a regulatory role in various diseases and tumors. LincRNA-p21 is a novel LncRNA that acts as a translational inhibitor by targeting mRNA or by directing the chromatin site of a protein-binding partner. LincRNA-p21 is closely related to a variety of tumors and exerts its biological functions of carcinogenesis or tumor suppression through different pathways. In this paper, the research progress of the tumor-associated gene LincRNA-p21 was reviews
ABSTRACT
OBJECTIVE: To investigate the effect of hyperthermia on the expression of PANDAR, LncRNA-p21 and ST8SIA genes in the human lung adenocarcinoma A549 cells. METHODS: A549 cells were randomly divided into 4 groups. The A549 cells in control group were cultured at 37 ℃; the cells at experimental groups were cultured at 40, 42 or 44 ℃ respectively. The cells in these 4 groups were incubated for 1 hour, and the levels of PANDAR, LncRNA-p21 and ST8SIA genes were analyzed by real-time fluorescence quantitative polymerase chain reaction. RESULTS: In cells cultured at 40, 42 or 44 ℃ experimental groups, the relative expression of PANDAR gene was lower than that of control group(P<0.05). In cells cultured at 44 ℃ experimental group, the relative expression of PANDAR gene was lower than that of the 40 and 42 ℃ experimental groups(P<0.05). There was no significant change in the relative expression of LncRNA-p21 and ST8SIA genes among the four groups(P>0.05). CONCLUSION: Hyperthermia decrease the expression of PANDAR gene in A549 cells.
ABSTRACT
Objective: To explore the mechanism of lncRNA-p21 regulating the apoptosis of cerebral smooth muscle cells (SMCs) involved in the occurrence and development of cerebral atherosclerosis. Methods: We selected 30 subjects receiving physical checkup and 30 patients with cerebrovascular atherosclerosis in our hospital. The content of lncRNA-p21 in serum was detected by Real-time fluorescence quantitative PCR. The rat model of cerebral atherosclerosis was established. The pathological changes of rat brain were observed by HE staining. The content of lncRNA-p21 in brain tissue was detected by RT-PCR; Western blot was used to detect the expressions of mTOR, anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax. Vascular smooth muscle cells (BVSMCs) were stimulated with ox-LDL to establish atherosclerosis model of vascular SMCs. lncRNA-p21 was overexpressed or inhibited. Apoptosis was detected by flow cytometry and TUNEL. The Caspase-3 activity assay kit was used to detect the expression of Caspase-3. Western Blot was used to detect the expressions of mTOR, anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax. Results: The expression of lncRNA-p21 was significantly decreased in patients with cerebral atherosclerosis and model group rats (P<0.05). HE staining showed that the decrease of lncRNA-p21 could contribute to the pathological changes of rat brain tissue accompanied by increased activity of Caspase-3, decreased expressions of Bcl-2 and mTOR protein and increased expression of Bax protein (P<0.05). Flow cytometry and TUNEL showed that overexpression of lncRNA-p21 could reduce the apoptosis of BVSMCs, inhibiting lncRNA-p21 could promote the apoptosis of BVSMCs (P<0.05). In VSMCs, lncRNA-p21 was overexpressed, Caspase-3 activity decreased, Bcl-2, mTOR protein expression increased, and Bax protein expression decreased (P<0.05). The expression of lncRNA-p21 was inhibited; Caspase-3 activity increased; Bcl-2 and mTOR protein expressions decreased; and Bax protein expression increased (P<0.05). Conclusion: lncRNA-p21 may regulate the apoptosis of cerebrovascular smooth muscle cells by regulating the expression of mTOR protein and participate in the pathological process of cerebral atherosclerosis.